Regulatory

Part:BBa_K4398000:Design

Designed by: Reinis Muiznieks   Group: iGEM22_Latvia-Riga   (2022-09-28)


PPGI promoter from Rhodotorula toruloides


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 272
    Illegal BglII site found at 487
    Illegal XhoI site found at 960
    Illegal XhoI site found at 1044
    Illegal XhoI site found at 1316
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1124
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Changed BsaI and SapI sites that were in the sequence by one nucleotide to allow for the use of RFC1000 standard



Source

https://onlinelibrary.wiley.com/doi/10.1002/yea.3145

References